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cd14 cell enrichment  (Miltenyi Biotec)


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    Miltenyi Biotec cd14 cell enrichment
    Cd14 Cell Enrichment, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 4235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd14 cell enrichment/product/Miltenyi Biotec
    Average 99 stars, based on 4235 article reviews
    cd14 cell enrichment - by Bioz Stars, 2026-03
    99/100 stars

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    Co-culturing experiments of M1 macrophages with stimulated Wharton’s Jelly and bone marrow-mesenchymal stromal cells. A: Schematic representation of the experimental workflow; B: Macrophage morphology was changed 9 d after co-culture either with Wharton’s Jelly or bone marrow-mesenchymal stromal cells. After 9 d, macrophages exhibited a more elongated shape and plastic adherence. Black arrows indicate the presence of elongated plastic adherent cells; C: Flow cytometry analysis showed the positive expression of CD45, CD14, and CD11b by the differentiated macrophages. After 9 d, the macrophages exhibited an increase in the CD29 and CD163 expression, compared to the cells at day 0. Statistically significant differences regarding the CD29 and CD163 were observed in M2 compared to M1 macrophages ( P < 0.001). BM: Bone marrow; MSCs: Mesenchymal stromal cells; WJ: Wharton’s Jelly; PBMCs: Peripheral blood mononuclear cells.

    Journal: World Journal of Biological Chemistry

    Article Title: Mesenchymal stromal cell delivery as a potential therapeutic strategy against COVID-19: Promising evidence from in vitro results

    doi: 10.4331/wjbc.v13.i2.47

    Figure Lengend Snippet: Co-culturing experiments of M1 macrophages with stimulated Wharton’s Jelly and bone marrow-mesenchymal stromal cells. A: Schematic representation of the experimental workflow; B: Macrophage morphology was changed 9 d after co-culture either with Wharton’s Jelly or bone marrow-mesenchymal stromal cells. After 9 d, macrophages exhibited a more elongated shape and plastic adherence. Black arrows indicate the presence of elongated plastic adherent cells; C: Flow cytometry analysis showed the positive expression of CD45, CD14, and CD11b by the differentiated macrophages. After 9 d, the macrophages exhibited an increase in the CD29 and CD163 expression, compared to the cells at day 0. Statistically significant differences regarding the CD29 and CD163 were observed in M2 compared to M1 macrophages ( P < 0.001). BM: Bone marrow; MSCs: Mesenchymal stromal cells; WJ: Wharton’s Jelly; PBMCs: Peripheral blood mononuclear cells.

    Article Snippet: Finally, the CD14+ cells were separated with negative selection using the human CD14+ cell enrichment (Stem Cell Technologies) according to the manufacturer’s instructions.

    Techniques: Co-Culture Assay, Flow Cytometry, Expressing